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We Beforehand recognized PS1 mutants Lively from the absence of NCT [29]. These mutants possessed two missense mutations in PS1: S438P with the ninth transmembrane area along with a single mutation distributed all over the molecule. These PS1 mutations Increased and modulated the γ-secretase action and reduced the ratio of long Aβs [32]. We then isolated Aph1 or Pen2 mutants ready to mature from the absence of NCT. right after we experienced screened cells with Pen2 or Aph1 mutations and the PS1 S438P mutation, we uncovered thirteen Aph1 mutants that grew inside the absence of NCT (Table 1 and Figure 1).
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